Laboratory Heading link

The ITR laboratory hosts the following facilities:

686 sq. ft.  68 ft. shower/change room

  • safety features: negative air flow, pass-through autoclave and ceiling mounted UV lights
  • Glas-Col aerosol infection chambers (2)
  • 4-ft. (2) and 6-ft. Baker SterilGard class II biosafety cabinets
  • CO2 incubators (2)
  • Reach-in incubators (2)
  • Centrifuge, Table top with biosafety rotor and cups
  • Anoxomat anaerobic environment generating system
  • BMG ClarioStar multilabel readers (2), Berthold microplate luminometer
  • GeneSys 30 spectrophotometer
  • BioRad Gene Pulser electroporator
  • -80C freezer
  • refrigerator

488 sq. ft. for microbial and cell culture

  • 4 ft. (2) and 6 ft. Baker SterilGard class II biosafety cabinets
  • 6 ft. Baker SterilChemGard class II biosafety cabinet
  • Baker EdgeGard clean bench for media prep
  • ClarioStar microplate multilabel reader
  • GeneSys 30 spectrophotometer
  • Leica DM IRB inverted microscope with image capture
  • Sorvall ST8 tabletop centrifuge
  • CO2 incubators (2)
  • Innova incubator shaker
  • BioRad Gene Pulser Xcell electroporator
  • Misonix 3000 cup horn sonicator
  • sonicator bath
Anteroom

153 sq. ft. serving both BSL-2 and BSL-3 laboratories.

  • Tuttnauer pass through double-door autoclave
  • Milli-Q water purification system
  • Panasonic vertical autoclave
  • 6′ refrigerator
QTOF

754 sq. ft. lab with two bays for synthesis

  • Bruker Impact II Q-TOF mass spectrometer & Shimadzu UHPLC
  • Agilent GC-MS (triple quadrapole)
  • Shimadzu semi-prep HPLC
  • Biotage solid phase extraction system
  • chemical fume hoods (3)

418 sq. ft. lab

  • FPLC, Aktapure
  • microscope, Leica
  • crystal incubator
  • plate reader (2)
  • thermal cycler (2)
  • gel electrophoresis (DNA protein)
  • gel documentation system
  • centrifuges (3), refrigerated
  • nanodrop spectrophotometer
  • incubator (2)
  • shaking incubator (2)
  • 4′ Baker SterilGard biosafety cabinet
  • -20 freezers (2)
  • refrigerator (2)

427 sq. ft. lab for isolation and culture of environmental microorganisms

  • shaker platforms (4) with total capacity for 45 2.8L Fernbach flasks or equivalent
  • 6′ Baker SterilGard class II biosafety cabinet
  • Sorvall 10L capacity refrigerated centrifuge
  • Eppendorf 5810R refrigerated centrifuge
  • -80C vertical freezers (2)
  • -30C vertical freezer
  • 6 ft. refrigerator
HPLC

 

  • Rotary evaporators with pumps and chillers (4)
  • Size exclusion, reversed-phased and normal-phased chromatography with fraction collectors
  • Shimadzu HPLC system with fraction collectors and UV-DAD detector supported for analytical, semi-preparative and preparative columns
  • High-performance thin layer chromatography (HPTLC) system with autosampler, developing chamber and visualizer
  • Solid phase extraction (SPE) unit
  • SpeedVac vacuum concentrator (2)
  • Analytical balance (2)
  • Freeze dryer (Lyophilizer)
  • pH meter
  • -20 oC freezer
  • Sonicator bath
  • Chemical fume hoods (3)

Other ITR Resources Heading link

Outside of the main ITR lab, other resources exist for users:

The Biological Resources Laboratory (BRL) at UIC is comprised of 104,000 sq. ft. on three levels. This includes 43 animal rooms for rodents and lagomorphs, each in a suite configuration with its own air handling system. Space is available for projects on an “as needed” basis. The ITR occupies a room with negative air pressure that is only used for infectious agents. It shares a 2nd room for uninfected mice which are used for tolerance and PK studies. Mice are housed in the BRL. Each floor of the BRL is managed by a team consisting of a Supervisor of Laboratory Animal Care, a board certified Laboratory Animal Veterinarian and a postdoctoral fellow. This management team oversees and works closely with a staff of certified laboratory animal care technicians. Working in collaboration with the investigators and their staff, the BRL staff provides support service for a wide variety of experimental protocols. The UIC Animal Care Program is accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and has consistently received positive evaluations from representatives of the Food and Drug Administration, the World Health Organization, the National Institutes of Health and several commercial pharmaceutical and contract laboratories.

Mass Spectrometry

The following LC-MS instruments are available through the UIC Research Resources Center and are located on the 5th floor of the College of Pharmacy.

Agilent 19646A LC-MSD Single Quad
Thermo TSQ Quantum Triple Quad
Waters Waters SYNAPT oa-Qtof SYNAPT
Shimadzu LCMS IT-TOF LCMS IT-TOF
Agilent 6410 QQQ Triple Quad
ABI Voyager-DE Pro MALDI-TOF
Thermo LTQ FT ICR LC
Thermo Orbitrap Velos Pro Orbitrap Velos Pro

NMR

The Research Resources Center has 360 and 500 MHz instruments available for sample submission and is located across the street in the Medical Sciences Building. The Department of Pharmaceutical Sciences also maintains two 300 MHz and one 400 MHz instruments on the 5th floor of the College of Pharmacy. ITR faculty have their academic appointments in this department and therefore are eligible to use these shared instruments. In addition the Center for Structural Biology, located one block from the College of Pharmacy has 600, 800 and 900 MHz NMR available for use.