Photo of Hyun Lee

Hyun Lee


INST AFFL, Institute for Tuberculosis Research
Director of Biophysics Core, Research Resources Center
Research Associate Professor, Pharmaceutical Sciences
Center for Biomolecular Sciences

Contact

Building & Room:

119 CFSB, MC 937

Address:

1100 S. Ashland Avenue, Chicago, IL, 60607

Office Phone:

(312) 355-5292

Email:

danielhl@uic.edu

CV Link:

Hyun Lee

Selected Publications

  • Son KN, Lee H, Shah D, Kalmodia S, Miller RC, Ali M, Balasubramaniam A, Cologna SM, Kong H, Shukla D, Aakalu VK. Histatin-1 is an Endogenous Ligand of the Sigma-2 Receptor.. The FEBS journal. 2021;. doi:10.1111/febs.16108.

  • Du R, Cooper L, Chen Z, Lee H, Rong L, Cui Q. Discovery of chebulagic acid and punicalagin as novel allosteric inhibitors of SARS-CoV-2 3CLpro.. Antiviral research. 2021;190:105075. doi:10.1016/j.antiviral.2021.105075.

  • Chen Z, Cui Q, Cooper L, Zhang P, Lee H, Chen Z, Wang Y, Liu X, Rong L, Du R. Ginkgolic acid and anacardic acid are specific covalent inhibitors of SARS-CoV-2 cysteine proteases.. Cell & bioscience. 2021;11(1):45. doi:10.1186/s13578-021-00564-x.

  • Youn I, Wu Z, Papa S, Burdette JE, Oyawaluja BO, Lee H, Che C. Limonoids and other triterpenoids from Entandrophragma angolense.. Fitoterapia. 2021;150:104846. doi:10.1016/j.fitote.2021.104846.

  • Schafer A, Xiong R, Cooper L, Nowar R, Lee H, Li Y, Ramirez BE, Peet NP, Caffrey M, Thatcher GRJ , Saphire EO, Cheng H, Rong L. Evidence for distinct mechanisms of small molecule inhibitors of filovirus entry.. PLoS pathogens. 2021;17(2):e1009312. doi:10.1371/journal.ppat.1009312.

  • Waters SB, Zhou C, Nguyen T, Zelkha R, Lee H, Kazlauskas A, Rosenblatt MI, Malik AB, Yamada KH. VEGFR2 Trafficking by KIF13B Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration.. Investigative ophthalmology & visual science. 2021;62(2):5. doi:10.1167/iovs.62.2.5.

  • Anirudhan V, Lee H, Cheng H, Cooper L, Rong L. Targeting SARS-CoV-2 viral proteases as a therapeutic strategy to treat COVID-19.. Journal of medical virology. 2021;. doi:10.1002/jmv.26814.

  • Roy S, Kapoor A, Zhu F, Mukhopadhyay R, Ghosh AK, Lee H, Mazzone J, Posner GH, Arav-Boger R. Artemisinins target the intermediate filament protein vimentin for human cytomegalovirus inhibition.. The Journal of biological chemistry. 2020;295(44):15013-15028. doi:10.1074/jbc.RA120.014116.

  • Wolf N, Lee H, Zagal D, Nam J, Oh DC, Lee H, Suh JW, Pauli GF, Cho S, Abad-Zapatero C. Structure of the N-terminal domain of ClpC1 in complex with the antituberculosis natural product ecumicin reveals unique binding interactions.. Acta crystallographica. Section D, Structural biology. 2020;76(Pt 5):458-471. doi:10.1107/S2059798320004027.

  • Zhou B, Shetye G, Yu Y, Santarsiero BD, Klein L, Abad-Zapatero C, Wolf N, Cheng J, Jin Y, Lee H, Suh JW, Lee H, Bisson J, McAlpine J, Chen S, Cho S, Franzblau SG, Pauli GF. Antimycobacterial Rufomycin Analogues from Streptomyces atratus Strain MJM3502.. Journal of natural products. 2020;83(3):657-667. doi:10.1021/acs.jnatprod.9b01095.

  • Ren J, Lee H, Kotak A, Johnson ME. MD simulations reveal alternate conformations of the oxyanion hole in the Zika virus NS2B/NS3 protease.. Proteins. 2020;88(2):345-354. doi:10.1002/prot.25809.

  • Lee H, Boyle-Vavra S, Ren J, Jarusiewicz J, Sharma LK, Hoagland DT, Yin S, Zhu T, Hevener KE, Ojeda I, Lee RE, Daum RS, Johnson ME. Identification of small molecules exhibiting oxacillin synergy through a novel assay for inhibition of vraTSR expression in methicillin resistant Staphylococcus aureus.. Antimicrobial agents and chemotherapy. 2019;. doi:10.1128/AAC.02593-18.

  • Ren J, Ojeda I, Patel M, Johnson ME, Lee H. Exploring small molecules with pan-genotypic inhibitory activities against hepatitis C virus NS3/4A serine protease.. Bioorganic & medicinal chemistry letters. 2019;. doi:10.1016/j.bmcl.2019.06.009.

  • Wolf N, Lee H, Choules MP, Pauli GF, Phansalkar RS, Anderson JR, Gao W, Ren J, Santarsiero BD, Lee H, Cheng J, Jin YY, Ho NA, Duc NM, Suh JW, Abad-Zapatero C, Cho S. High-Resolution Structure of ClpC1-Rufomycin and Ligand Binding Studies Provide a Framework to Design and Optimize Anti-Tuberculosis Leads.. ACS infectious diseases. 2019;. doi:10.1021/acsinfecdis.8b00276.

  • Lee H, Ren J, Pesavento R, Ojeda I, Rice AJ, Lv H, Kwon Y, Johnson ME. Identification and design of novel small molecule inhibitors against MERS-CoV papain-like protease via high-throughput screening and molecular modeling.. Bioorganic & medicinal chemistry. 2019;. doi:10.1016/j.bmc.2019.03.050.

  • Choules MP, Wolf N, Lee H, Anderson JR, Grzelak E, Wang Y, Ma R, Gao W, McAlpine JB, Jin YY, Cheng J, Lee H, Suh JW, Duc NM, Paik S, Choe JH, Jo EK, Chang CL, Lee J, Jaki BU, Pauli GF, Franzblau SG, Cho S. Rufomycin Targets ClpC1 Proteolysis in Mycobacterium tuberculosis and M. abscessus.. Antimicrobial agents and chemotherapy. 2019;63(3). doi:10.1128/AAC.02204-18.

  • Koh KH, Cao Y, Mangos S, Tardi NJ, Dande RR, Lee HW, Samelko B, Altintas MM, Schmitz VP, Lee H, Mukherjee K, Peev V, Cimbaluk DJ, Reiser J, Hahm E. Non-immune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist.. The Journal of clinical investigation. 2019;. doi:10.1172/JCI123386.

  • Oguri T, Kwon Y, Woo JKK , Prehna G, Lee H, Ning M, Won K, Lee J, Mei S, Shi Y, Jeong H, Lee H. A family of small intrinsically disordered proteins involved in flagella-dependent motility in Salmonella.. Journal of bacteriology. 2018;. doi:10.1128/JB.00415-18.

  • Ren J, Dsouza NR, Deng H, Lee H, Bouvier M, Johnson ME. Discovery of small molecule inhibitors of adenovirus by disrupting E3-19K/HLA-A2 interactions.. Bioorganic & medicinal chemistry letters. 2018;. doi:10.1016/j.bmcl.2018.07.036.

  • Moon HG, Kim SJ, Jeong JJ, Han SS, Jarjour NN, Lee H, Abboud-Werner SL, Chung S, Choi HS, Natarajan V, Ackerman SJ, Christman JW, Park GY. Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization.. Immunity. 2018;. doi:10.1016/j.immuni.2018.06.009.

  • Hevener KE, Santarsiero BD, Lee H, Jones JA, Boci T, Johnson ME, Mehboob S. Structural characterization of Porphyromonas gingivalis enoyl-ACP reductase II (FabK).. Acta crystallographica. Section F, Structural biology communications. 2018;74(Pt 2):105-112. doi:10.1107/S2053230X18000262.

  • Hevener KE, Pesavento R, Ren J, Lee H, Ratia KM, Johnson ME. Hit-to-Lead: Hit Validation and Assessment.. Methods in enzymology. 2018;610:265-309. doi:10.1016/bs.mie.2018.09.022.

  • Holman KM, Puppala AK, Lee J, Lee H, Simonovic M. Insights into substrate promiscuity of human seryl-tRNA synthetase.. RNA (New York, N.Y.). 2017;. doi:10.1261/rna.061069.117.

  • Hayek SS, Koh KH, Grams ME, Wei C, Ko YA, Li J, Samelko B, Lee H, Dande RR, Lee HW, Hahm E, Peev V, Tracy M, Tardi NJ, Gupta V, Altintas MM, Garborcauskas G, Stojanovic N, Winkler CA, Lipkowitz MS, Tin A, Inker LA, Levey AS, Zeier M, Freedman BI, Kopp JB, Skorecki K, Coresh J, Quyyumi AA, Sever S, Reiser J. A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease.. Nature medicine. 2017;. doi:10.1038/nm.4362.

  • Lee H, Ren J, Nocadello S, Rice AJ, Ojeda I, Light S, Minasov G, Vargas J, Nagarathnam D, Anderson WF, Johnson ME. Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from Zika virus.. Antiviral research. 2016;. doi:10.1016/j.antiviral.2016.12.016.

  • Rice AJ, Lei H, Santarsiero BD, Lee H, Johnson ME. Ca-asp bound X-ray structure and inhibition of Bacillus anthracis dihydroorotase (DHOase).. Bioorganic & medicinal chemistry. 2016;. doi:10.1016/j.bmc.2016.07.055.

  • Han KY, Chang JH, Lee H, Azar DT. Proangiogenic Interactions of Vascular Endothelial MMP14 With VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis.. Investigative ophthalmology & visual science. 2016;57(7):3313-22. doi:10.1167/iovs.16-19420.

  • Lei H, Jones C, Zhu T, Patel K, Wolf NM, Fung LW, Lee H, Johnson ME. Identification of B. anthracis N5-carboxyaminoimidazole ribonucleotide mutase (PurE) active site binding compounds via fragment library screening.. Bioorganic & medicinal chemistry. 2015;. doi:10.1016/j.bmc.2015.12.029.

  • Jain AD, Potteti H, Richardson BG, Kingsley L, Luciano JP, Ryuzoji AF, Lee H, Krunic A, Mesecar AD, Reddy SP, Moore T. Probing the structural requirements of non-electrophilic naphthalene-based Nrf2 activators.. European journal of medicinal chemistry. 2015;103:252-68. doi:10.1016/j.ejmech.2015.08.049.

  • Rice AJ, Woo JK, Khan A, Szypulinski MZ, Johnson ME, Lee H, Lee H. Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR.. Protein expression and purification. 2015;. doi:10.1016/j.pep.2015.08.030.

  • Han KY, Dugas-Ford J, Lee H, Chang JH, Azar DT. MMP14 Cleavage of VEGFR1 in the Cornea Leads to a VEGF-Trap Antiangiogenic Effect.. Investigative ophthalmology & visual science. 2015;56(9):5450-6. doi:10.1167/iovs.14-16248.

  • Lee H, Lei HD, Santarsiero BL, Gatuz JJ, Cao SZ, Rice AK, Patel KE, Szypulinski M, Ojeda I, Ghosh A, Johnson M. Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV.. ACS chemical biology. 2015;10(6):1456-65. doi:10.1021/cb500917m.

  • Aggarwal CC, Jimenez Romaguera J, Lee HE, Chlipala G, Ratia K, Federle M. Identification of Quorum-Sensing Inhibitors Disrupting Signaling between Rgg and Short Hydrophobic Peptides in Streptococci.. mBio. 2015;6(3):e00393-15. doi:10.1128/mBio.00393-15.

  • Han KY, Chang M, Ying HY, Lee H, Huang YH, Chang JH, Azar DT. Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro.. Protein and peptide letters. 2015;22(11):1025-30.

  • Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC, Johnson ME. Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings.. Bioorganic & medicinal chemistry. 2014;22(1):167-77. doi:10.1016/j.bmc.2013.11.041.

  • Ratia K, Mehboob S, Lee H. Ligand screening using enzymatic assays.. Methods in molecular biology (Clifton, N.J.). 2014;1140:291-304. doi:10.1007/978-1-4939-0354-2_21.

  • Rice AJ, Truong L, Johnson ME, Lee H. A colorimetric assay optimization for high-throughput screening of dihydroorotase by detecting ureido groups.. Analytical biochemistry. 2013;441(1):87-94. doi:10.1016/j.ab.2013.05.035.

  • Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, Johnson ME. Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications.. ChemMedChem. 2013;8(8):1361-72. doi:10.1002/cmdc.201300134.

  • Zhu T, Lee H, Lei H, Jones C, Patel K, Johnson ME, Hevener KE. Fragment-based drug discovery using a multidomain, parallel MD-MM/PBSA screening protocol.. Journal of chemical information and modeling. 2013;53(3):560-72. doi:10.1021/ci300502h.

  • Truong L, Hevener KE, Rice AJ, Patel K, Johnson ME, Lee H. High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion.. Protein expression and purification. 2013;88(1):98-106. doi:10.1016/j.pep.2012.11.018.

  • Lee H, Zhu TY, Patel KE, Zhang YL, Truong L, Hevener KL, Gatuz JE, Subramanya G, Jeong H, Uprichard S, Johnson M. High-throughput screening (HTS) and hit validation to identify small molecule inhibitors with activity against NS3/4A proteases from multiple hepatitis C virus genotypes.. PloS one. 2013;8(10):e75144. doi:10.1371/journal.pone.0075144.

  • Han KY, Azar DT, Sabri A, Lee H, Jain S, Lee BS, Chang JH. Characterization of the interaction between endostatin short peptide and VEGF receptor 3.. Protein and peptide letters. 2012;19(9):969-74.

  • Chaudhuri R, Lee H, Truong L, Torres J, Patel K, Johnson ME. Identification of non-macrocyclic small molecule inhibitors against the NS3/4A serine protease of hepatitis C virus through in silico screening.. Journal of chemical information and modeling. 2012;52(8):2245-56. doi:10.1021/ci300177p.

  • Lee H, Torres J, Truong L, Chaudhuri R, Mittal A, Johnson ME. Reducing agents affect inhibitory activities of compounds: results from multiple drug targets.. Analytical biochemistry. 2012;423(1):46-53. doi:10.1016/j.ab.2012.01.006.

  • Ghosh AK, Takayama J, Rao KV, Ratia K, Chaudhuri R, Mulhearn DC, Lee H, Nichols DB, Baliji S, Baker SC, Johnson ME, Mesecar AD. Severe acute respiratory syndrome coronavirus papain-like novel protease inhibitors: design, synthesis, protein-ligand X-ray structure and biological evaluation.. Journal of medicinal chemistry. 2010;53(13):4968-79. doi:10.1021/jm1004489.

  • Mahajan A, Yuan C, Lee H, Chen ES, Wu PY, Tsai MD. Structure and function of the phosphothreonine-specific FHA domain.. Science signaling. 2008;1(51):re12. doi:10.1126/scisignal.151re12.

  • Lee H, Yuan C, Hammet A, Mahajan A, Chen ES, Wu MR, Su MI, Heierhorst J, Tsai MD. Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade.. Molecular cell. 2008;30(6):767-78. doi:10.1016/j.molcel.2008.05.013.

  • Qin D, Lee H, Yuan C, Ju Y, Tsai MD. Identification of potential binding sites for the FHA domain of human Chk2 by in vitro binding studies.. Biochemical and biophysical research communications. 2003;311(4):803-8.

  • Lee H, Mok KH, Muhandiram R, Park KH, Suk JE, Kim DH, Chang J, Sung YC, Choi KY, Han KH. Local structural elements in the mostly unstructured transcriptional activation domain of human p53.. The Journal of biological chemistry. 2000;275(38):29426-32. doi:10.1074/jbc.M003107200.

    • Education

  • PhD, Biophysics, The Ohio State University, Columbus, Ohio

  • MS, Chemistry, Chonnam National University, Kwangju, South Korea

  • BS, Agricultural Biology, Chonnam National University, Kwangju, South Korea